The groups were rescued in extremely black conditions by RNLI after two small boat accidents in the canal.
A Sudanese man in his twenties was found, on Friday, unconscious and pulled out of the water after overtaking a boat as he and others attempted to reach the UK, according to French authorities.
The man was brought back to the beach but pronounced dead.
The public prosecutor in Boulogne-sur-Mer said an investigation had been opened into the manslaughter.
In this incident, a total of 32 people were rescued off Berke, near Calais, in a state of hypothermia, authorities said.
The death came less than two months after at least 27 people died when their boat sank off the coast of France in November.
More than 450 people made the life-threatening journey across the canal in small boats in 2022, after a record-breaking year in 2021.
At least 271 people on 10 small boats arrived in the UK on Thursday, including a young boy in an orange life jacket and beanie.